Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells
An acidic phospholipase A2 (LPLA2) was recently purified and cloned. THP-1 cells were used to characterize the gene induction of LPLA2. THP-1 cells were stimulated with several differentiation agents. The LPLA2 mRNA and activity increased in cells treated with phorbol ester but not with vitamin D3,...
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| Format: | Article |
| Language: | English |
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Elsevier
2004-04-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520318514 |
| _version_ | 1818602160420552704 |
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| author | Akira Abe Heather K. Poucher Miki Hiraoka James A. Shayman |
| author_facet | Akira Abe Heather K. Poucher Miki Hiraoka James A. Shayman |
| author_sort | Akira Abe |
| collection | DOAJ |
| description | An acidic phospholipase A2 (LPLA2) was recently purified and cloned. THP-1 cells were used to characterize the gene induction of LPLA2. THP-1 cells were stimulated with several differentiation agents. The LPLA2 mRNA and activity increased in cells treated with phorbol ester but not with vitamin D3, interferon-γ, or granulocyte macrophage colony-stimulating factor. All-trans-retinoic acid enhanced mRNA expression and enzyme activity in a dose- and time-dependent manner. The natural 9-cis and 13-cis isomers of retinoic acid enhanced transcription and activity. Two classes of nuclear receptors, the retinoic acid receptor (RAR) and the retinoid X receptor (RXR), mediate retinoic acid signaling. Specific RAR and RXR agonists were used to identify the nuclear receptor responsible for LPLA2 induction by retinoic acid. Treatment with the RAR agonist 4-[E-2-tetrahydro-5,5,8,8-tetra-methyl-2-naphthalenyl]1-propenyl benzoic acid (TTNPB) resulted in a small and statistically significant increase of the mRNA expression and activity of LPLA2. The RXR agonist methoprene acid worked as well as all-trans-retinoic acid at increasing both mRNA and enzyme activity. The methoprene acid and TTNPB effects were not synergistic. The peroxisome proliferator-activated receptor γ agonists 15-deoxy-Δ12,14-prostaglandin J2 and troglitazone failed to induce LPLA2 activity and mRNA.Thus, an RXR-dependent pathway controls LPLA2 gene activation by retinoic acid in THP-1 cells. |
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| format | Article |
| id | doaj.art-9bde993b179a48cc920897b5c852d2cf |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T13:02:52Z |
| publishDate | 2004-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-9bde993b179a48cc920897b5c852d2cf2022-12-21T22:30:47ZengElsevierJournal of Lipid Research0022-22752004-04-01454667673Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cellsAkira Abe0Heather K. Poucher1Miki Hiraoka2James A. Shayman3Nephrology Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109Nephrology Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109Nephrology Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109Nephrology Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109An acidic phospholipase A2 (LPLA2) was recently purified and cloned. THP-1 cells were used to characterize the gene induction of LPLA2. THP-1 cells were stimulated with several differentiation agents. The LPLA2 mRNA and activity increased in cells treated with phorbol ester but not with vitamin D3, interferon-γ, or granulocyte macrophage colony-stimulating factor. All-trans-retinoic acid enhanced mRNA expression and enzyme activity in a dose- and time-dependent manner. The natural 9-cis and 13-cis isomers of retinoic acid enhanced transcription and activity. Two classes of nuclear receptors, the retinoic acid receptor (RAR) and the retinoid X receptor (RXR), mediate retinoic acid signaling. Specific RAR and RXR agonists were used to identify the nuclear receptor responsible for LPLA2 induction by retinoic acid. Treatment with the RAR agonist 4-[E-2-tetrahydro-5,5,8,8-tetra-methyl-2-naphthalenyl]1-propenyl benzoic acid (TTNPB) resulted in a small and statistically significant increase of the mRNA expression and activity of LPLA2. The RXR agonist methoprene acid worked as well as all-trans-retinoic acid at increasing both mRNA and enzyme activity. The methoprene acid and TTNPB effects were not synergistic. The peroxisome proliferator-activated receptor γ agonists 15-deoxy-Δ12,14-prostaglandin J2 and troglitazone failed to induce LPLA2 activity and mRNA.Thus, an RXR-dependent pathway controls LPLA2 gene activation by retinoic acid in THP-1 cells.http://www.sciencedirect.com/science/article/pii/S0022227520318514retinoic acidlysosomephospholipasephorbol ester |
| spellingShingle | Akira Abe Heather K. Poucher Miki Hiraoka James A. Shayman Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells Journal of Lipid Research retinoic acid lysosome phospholipase phorbol ester |
| title | Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells |
| title_full | Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells |
| title_fullStr | Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells |
| title_full_unstemmed | Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells |
| title_short | Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells |
| title_sort | induction of lysosomal phospholipase a2 through the retinoid x receptor in thp 1 cells |
| topic | retinoic acid lysosome phospholipase phorbol ester |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520318514 |
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