Characterization of multiple interactions between the envelope E protein of SARS-CoV-2 and human BRD4

Characterization of multiple interactions between the envelope E protein of SARS-CoV-2 and human BRD4

Summary: The SARS-CoV-2 envelope (E) protein hijacks human BRD4 (bromodomain and extra-terminal domain protein 4). Here, we describe a protocol to characterize the interaction of the acetylated E protein with BRD4 in vivo. We detail steps to use NMR spectroscopy to map the binding interface and incl...

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Bibliographic Details
Main Authors: Mohamad Zandian, Suk Min Jang, Catherine Lachance, Arpan Acharya, Siddappa N. Byrareddy, Jacques Côté, Tatiana G. Kutateladze
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Microbiology
Molecular Biology
Protein Biochemistry
Structural Biology
NMR
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200733X

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http://www.sciencedirect.com/science/article/pii/S266616672200733X

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