Effect of sequence depth and length in long-read assembly of the maize inbred NC358

Effect of sequence depth and length in long-read assembly of the maize inbred NC358

Sequence depth and read length determine the quality of genome assembly. Here, the authors leverage a set of PacBio reads to develop guidelines for sequencing and assembly of complex plant genomes in order to allocate finite resources using maize as an example.

Bibliographic Details
Main Authors: Shujun Ou, Jianing Liu, Kapeel M. Chougule, Arkarachai Fungtammasan, Arun S. Seetharam, Joshua C. Stein, Victor Llaca, Nancy Manchanda, Amanda M. Gilbert, Sharon Wei, Chen-Shan Chin, David E. Hufnagel, Sarah Pedersen, Samantha J. Snodgrass, Kevin Fengler, Margaret Woodhouse, Brian P. Walenz, Sergey Koren, Adam M. Phillippy, Brett T. Hannigan, R. Kelly Dawe, Candice N. Hirsch, Matthew B. Hufford, Doreen Ware
Format: Article
Language:English
Published: Nature Portfolio 2020-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-16037-7

Internet

https://doi.org/10.1038/s41467-020-16037-7

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