Heterologous expression of a novel linoleic acid isomerase BBI, and effect of fusion tags on its performance

Heterologous expression of a novel linoleic acid isomerase BBI, and effect of fusion tags on its performance

Functional proteins with the ability to isomerise free linoleic acid (LA) to conjugated linoleic acid (CLA) are termed linoleic acid isomerases (LAI). BBI is a novel LAI from Bifidobacterium breve with unique advantages in the production of a single CLA isomer; however, its complex membrane-bound fo...

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Bibliographic Details
Main Authors: Yongchao Mei, Xiuqing Li, Bo Yang, Jianxin Zhao, Hao Zhang, Haiqin Chen, Wei Chen
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:Current Research in Food Science
Subjects:
Conjugated linoleic acid (CLA)
Membrane protein
Bifidobacterium breve
C-terminal His-tag
CLA isomers
De novo protein modelling
Online Access:http://www.sciencedirect.com/science/article/pii/S2665927122001162

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http://www.sciencedirect.com/science/article/pii/S2665927122001162

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