One-step generation of error-prone PCR libraries using Gateway<sup>® </sup>technology

<p>Abstract</p> <p>Background</p> <p>Error-prone PCR (epPCR) libraries are one of the tools used in directed evolution. The Gateway<sup>® </sup>technology allows constructing epPCR libraries virtually devoid of any background (<it>i.e</it>., of i...

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Bibliographic Details
Main Authors: Gruet Antoine, Longhi Sonia, Bignon Christophe
Format: Article
Language:English
Published: BMC 2012-01-01
Series:Microbial Cell Factories
Subjects:
Online Access:http://www.microbialcellfactories.com/content/11/1/14