One-step generation of error-prone PCR libraries using Gateway<sup>® </sup>technology
<p>Abstract</p> <p>Background</p> <p>Error-prone PCR (epPCR) libraries are one of the tools used in directed evolution. The Gateway<sup>® </sup>technology allows constructing epPCR libraries virtually devoid of any background (<it>i.e</it>., of i...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2012-01-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | http://www.microbialcellfactories.com/content/11/1/14 |