Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A Gene
<i>Leuconostoc</i> spp. are environmental microorganisms commonly associated with fermented foods. Absence of antibiotic resistance (AR) in bacteria is a critical issue for global food safety. Herein, we updated the occurrence of AR genes in the <i>Leuconostoc</i> genus throu...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/3/491 |
| _version_ | 1797395174228230144 |
|---|---|
| author | Elisa Salvetti Ilenia Campedelli Ilaria Larini Giada Conedera Sandra Torriani |
| author_facet | Elisa Salvetti Ilenia Campedelli Ilaria Larini Giada Conedera Sandra Torriani |
| author_sort | Elisa Salvetti |
| collection | DOAJ |
| description | <i>Leuconostoc</i> spp. are environmental microorganisms commonly associated with fermented foods. Absence of antibiotic resistance (AR) in bacteria is a critical issue for global food safety. Herein, we updated the occurrence of AR genes in the <i>Leuconostoc</i> genus through in silico analyses of the genomes of 17 type strains. A total of 131 putative AR traits associated with the main clinically relevant antibiotics were detected. We found, for the first time, the <i>lsa</i>A gene in <i>L. fallax</i> ATCC 700006<sup>T</sup> and <i>L. pseudomesenteroides</i> NCDO 768<sup>T</sup>. Their amino acid sequences displayed high similarities (59.07% and 52.21%) with LsaA of <i>Enterococcus</i><i>faecalis</i> V583, involved in clindamycin (CLI) and quinupristin-dalfopristin (QUD) resistance. This trait has different distribution patterns in <i>Leuconostoc</i> nontype strains—i.e., <i>L. pseudomesenteroides</i>, <i>L. lactis</i> and <i>L. falkenbergense</i> isolates from fermented vegetables, cheeses, and starters. To better explore the role of <i>lsa</i>A, MIC for CLI and QUD were assessed in ATCC 700006<sup>T</sup> and NCDO 768<sup>T</sup>; both strains were resistant towards CLI, potentially linking <i>lsa</i>A to their resistant phenotype. Contrarily, NCDO 768<sup>T</sup> was sensitive towards QUD; however, expression of <i>lsa</i>A increased in presence of this antibiotic, indicating an active involvement of this trait and thus suggesting a revision of the QUD thresholds for this species. |
| first_indexed | 2024-03-09T00:30:40Z |
| format | Article |
| id | doaj.art-9c636e93e8264e29b315b1b7fd0fc832 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T00:30:40Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9c636e93e8264e29b315b1b7fd0fc8322023-12-11T18:33:43ZengMDPI AGMicroorganisms2076-26072021-02-019349110.3390/microorganisms9030491Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A GeneElisa Salvetti0Ilenia Campedelli1Ilaria Larini2Giada Conedera3Sandra Torriani4Department of Biotechnology, University of Verona, 37134 Verona, ItalyMicrobion srl, San Giovanni Lupatoto, 37057 Verona, ItalyDepartment of Biotechnology, University of Verona, 37134 Verona, ItalyDepartment of Biotechnology, University of Verona, 37134 Verona, ItalyDepartment of Biotechnology, University of Verona, 37134 Verona, Italy<i>Leuconostoc</i> spp. are environmental microorganisms commonly associated with fermented foods. Absence of antibiotic resistance (AR) in bacteria is a critical issue for global food safety. Herein, we updated the occurrence of AR genes in the <i>Leuconostoc</i> genus through in silico analyses of the genomes of 17 type strains. A total of 131 putative AR traits associated with the main clinically relevant antibiotics were detected. We found, for the first time, the <i>lsa</i>A gene in <i>L. fallax</i> ATCC 700006<sup>T</sup> and <i>L. pseudomesenteroides</i> NCDO 768<sup>T</sup>. Their amino acid sequences displayed high similarities (59.07% and 52.21%) with LsaA of <i>Enterococcus</i><i>faecalis</i> V583, involved in clindamycin (CLI) and quinupristin-dalfopristin (QUD) resistance. This trait has different distribution patterns in <i>Leuconostoc</i> nontype strains—i.e., <i>L. pseudomesenteroides</i>, <i>L. lactis</i> and <i>L. falkenbergense</i> isolates from fermented vegetables, cheeses, and starters. To better explore the role of <i>lsa</i>A, MIC for CLI and QUD were assessed in ATCC 700006<sup>T</sup> and NCDO 768<sup>T</sup>; both strains were resistant towards CLI, potentially linking <i>lsa</i>A to their resistant phenotype. Contrarily, NCDO 768<sup>T</sup> was sensitive towards QUD; however, expression of <i>lsa</i>A increased in presence of this antibiotic, indicating an active involvement of this trait and thus suggesting a revision of the QUD thresholds for this species.https://www.mdpi.com/2076-2607/9/3/491<i>Leuconostoc</i>food chaingenomesantibiotic resistance<i>lsa</i>A geneclindamycin |
| spellingShingle | Elisa Salvetti Ilenia Campedelli Ilaria Larini Giada Conedera Sandra Torriani Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A Gene Microorganisms <i>Leuconostoc</i> food chain genomes antibiotic resistance <i>lsa</i>A gene clindamycin |
| title | Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A Gene |
| title_full | Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A Gene |
| title_fullStr | Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A Gene |
| title_full_unstemmed | Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A Gene |
| title_short | Exploring Antibiotic Resistance Diversity in <i>Leuconostoc</i> spp. by a Genome-Based Approach: Focus on the <i>lsa</i>A Gene |
| title_sort | exploring antibiotic resistance diversity in i leuconostoc i spp by a genome based approach focus on the i lsa i a gene |
| topic | <i>Leuconostoc</i> food chain genomes antibiotic resistance <i>lsa</i>A gene clindamycin |
| url | https://www.mdpi.com/2076-2607/9/3/491 |
| work_keys_str_mv | AT elisasalvetti exploringantibioticresistancediversityinileuconostocisppbyagenomebasedapproachfocusontheilsaiagene AT ileniacampedelli exploringantibioticresistancediversityinileuconostocisppbyagenomebasedapproachfocusontheilsaiagene AT ilarialarini exploringantibioticresistancediversityinileuconostocisppbyagenomebasedapproachfocusontheilsaiagene AT giadaconedera exploringantibioticresistancediversityinileuconostocisppbyagenomebasedapproachfocusontheilsaiagene AT sandratorriani exploringantibioticresistancediversityinileuconostocisppbyagenomebasedapproachfocusontheilsaiagene |