Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines
Verticillium wilt (caused by the pathogen Verticillium dahliae) is of high concern for cotton producers and consumers. The major strategy for controlling this disease is the development of resistant cotton (Gossypium spp.) cultivars. We used interspecific chromosome segment introgression lines (CSIL...
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KeAi Communications Co., Ltd.
2014-10-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514114000592 |
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| author | Peng Wang Zhiyuan Ning Ling Lin Hong Chen Hongxian Mei Jun Zhao Bingliang Liu Xin Zhang Wangzhen Guo Tianzhen Zhang |
| author_facet | Peng Wang Zhiyuan Ning Ling Lin Hong Chen Hongxian Mei Jun Zhao Bingliang Liu Xin Zhang Wangzhen Guo Tianzhen Zhang |
| author_sort | Peng Wang |
| collection | DOAJ |
| description | Verticillium wilt (caused by the pathogen Verticillium dahliae) is of high concern for cotton producers and consumers. The major strategy for controlling this disease is the development of resistant cotton (Gossypium spp.) cultivars. We used interspecific chromosome segment introgression lines (CSILs) to identify quantitative trait loci (QTL) associated with resistance to Verticillium wilt in cotton grown in greenhouse and inoculated with three defoliating V. dahliae isolates. A total of 42 QTL, including 23 with resistance-increasing and 19 with resistance-decreasing, influenced host resistance against the three isolates. These QTL were identified and mapped on 18 chromosomes (chromosomes A1, A3, A4, A5, A7, A8, A9, A12, A13, D1, D2, D3, D4, D5, D7, D8, D11, and D12), with LOD values ranging from 3.00 to 9.29. Among the positive QTL with resistance-increasing effect, 21 conferred resistance to only one V. dahliae isolate, suggesting that resistance to V. dahliae conferred by most QTL is pathogen isolate-specific. The At subgenome of cotton had greater effect on resistance to Verticillium wilt than the Dt subgenome. We conclude that pyramiding different resistant QTL could be used to breed cotton cultivars with broad-spectrum resistance to Verticillium wilt. |
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| id | doaj.art-9ccec3a5d1e74f82bd2e1808051e5089 |
| institution | Directory Open Access Journal |
| issn | 2095-5421 2214-5141 |
| language | English |
| last_indexed | 2024-12-17T20:28:20Z |
| publishDate | 2014-10-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Crop Journal |
| spelling | doaj.art-9ccec3a5d1e74f82bd2e1808051e50892022-12-21T21:33:40ZengKeAi Communications Co., Ltd.Crop Journal2095-54212214-51412014-10-0125278288doi:10.1016/j.cj.2014.06.007Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression linesPeng Wang0Zhiyuan Ning1Ling Lin2Hong Chen3Hongxian Mei 4Jun Zhao 5Bingliang Liu 6Xin Zhang 7Wangzhen Guo 8Tianzhen Zhang 9National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaCotton Research Institute, Xinjiang Academy of Agriculture and Reclamation Sciences, Xinjiang 832000, ChinaNational Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaVerticillium wilt (caused by the pathogen Verticillium dahliae) is of high concern for cotton producers and consumers. The major strategy for controlling this disease is the development of resistant cotton (Gossypium spp.) cultivars. We used interspecific chromosome segment introgression lines (CSILs) to identify quantitative trait loci (QTL) associated with resistance to Verticillium wilt in cotton grown in greenhouse and inoculated with three defoliating V. dahliae isolates. A total of 42 QTL, including 23 with resistance-increasing and 19 with resistance-decreasing, influenced host resistance against the three isolates. These QTL were identified and mapped on 18 chromosomes (chromosomes A1, A3, A4, A5, A7, A8, A9, A12, A13, D1, D2, D3, D4, D5, D7, D8, D11, and D12), with LOD values ranging from 3.00 to 9.29. Among the positive QTL with resistance-increasing effect, 21 conferred resistance to only one V. dahliae isolate, suggesting that resistance to V. dahliae conferred by most QTL is pathogen isolate-specific. The At subgenome of cotton had greater effect on resistance to Verticillium wilt than the Dt subgenome. We conclude that pyramiding different resistant QTL could be used to breed cotton cultivars with broad-spectrum resistance to Verticillium wilt.http://www.sciencedirect.com/science/article/pii/S2214514114000592Resistance; QTLChromosomePyramidingGossypium hirsutumGossypium barbadense |
| spellingShingle | Peng Wang Zhiyuan Ning Ling Lin Hong Chen Hongxian Mei Jun Zhao Bingliang Liu Xin Zhang Wangzhen Guo Tianzhen Zhang Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines Crop Journal Resistance; QTL Chromosome Pyramiding Gossypium hirsutum Gossypium barbadense |
| title | Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines |
| title_full | Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines |
| title_fullStr | Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines |
| title_full_unstemmed | Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines |
| title_short | Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines |
| title_sort | genetic dissection of tetraploid cotton resistant to verticillium wilt using interspecific chromosome segment introgression lines |
| topic | Resistance; QTL Chromosome Pyramiding Gossypium hirsutum Gossypium barbadense |
| url | http://www.sciencedirect.com/science/article/pii/S2214514114000592 |
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