Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase
Abstract The nucleus plays a central role in several key cellular processes, including chromosome organisation, DNA replication and gene transcription. Recent work suggests an association between nuclear mechanics and cell-cycle progression, but many aspects of this connection remain unexplored. Her...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-05074-9 |
| _version_ | 1827899708535734272 |
|---|---|
| author | Viola Introini Gururaj Rao Kidiyoor Giancarlo Porcella Pietro Cicuta Marco Cosentino Lagomarsino |
| author_facet | Viola Introini Gururaj Rao Kidiyoor Giancarlo Porcella Pietro Cicuta Marco Cosentino Lagomarsino |
| author_sort | Viola Introini |
| collection | DOAJ |
| description | Abstract The nucleus plays a central role in several key cellular processes, including chromosome organisation, DNA replication and gene transcription. Recent work suggests an association between nuclear mechanics and cell-cycle progression, but many aspects of this connection remain unexplored. Here, by monitoring nuclear shape fluctuations at different cell cycle stages, we uncover increasing inward fluctuations in late G2 and in early prophase, which are initially transient, but develop into instabilities when approaching the nuclear-envelope breakdown. We demonstrate that such deformations correlate with chromatin condensation by perturbing both the chromatin and the cytoskeletal structures. We propose that the contrasting forces between an extensile stress and centripetal pulling from chromatin condensation could mechanically link chromosome condensation with nuclear-envelope breakdown, two main nuclear processes occurring during mitosis. |
| first_indexed | 2024-03-12T23:21:31Z |
| format | Article |
| id | doaj.art-9d09cc520b4449ccbf38b9c49187eba3 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-12T23:21:31Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-9d09cc520b4449ccbf38b9c49187eba32023-07-16T11:25:02ZengNature PortfolioCommunications Biology2399-36422023-07-016111110.1038/s42003-023-05074-9Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophaseViola Introini0Gururaj Rao Kidiyoor1Giancarlo Porcella2Pietro Cicuta3Marco Cosentino Lagomarsino4Cavendish Laboratory, University of CambridgeIFOM, FIRC Institute of Molecular OncologyIFOM, FIRC Institute of Molecular OncologyCavendish Laboratory, University of CambridgeIFOM, FIRC Institute of Molecular OncologyAbstract The nucleus plays a central role in several key cellular processes, including chromosome organisation, DNA replication and gene transcription. Recent work suggests an association between nuclear mechanics and cell-cycle progression, but many aspects of this connection remain unexplored. Here, by monitoring nuclear shape fluctuations at different cell cycle stages, we uncover increasing inward fluctuations in late G2 and in early prophase, which are initially transient, but develop into instabilities when approaching the nuclear-envelope breakdown. We demonstrate that such deformations correlate with chromatin condensation by perturbing both the chromatin and the cytoskeletal structures. We propose that the contrasting forces between an extensile stress and centripetal pulling from chromatin condensation could mechanically link chromosome condensation with nuclear-envelope breakdown, two main nuclear processes occurring during mitosis.https://doi.org/10.1038/s42003-023-05074-9 |
| spellingShingle | Viola Introini Gururaj Rao Kidiyoor Giancarlo Porcella Pietro Cicuta Marco Cosentino Lagomarsino Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase Communications Biology |
| title | Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase |
| title_full | Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase |
| title_fullStr | Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase |
| title_full_unstemmed | Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase |
| title_short | Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase |
| title_sort | centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase |
| url | https://doi.org/10.1038/s42003-023-05074-9 |
| work_keys_str_mv | AT violaintroini centripetalnuclearshapefluctuationsassociatewithchromatincondensationinearlyprophase AT gururajraokidiyoor centripetalnuclearshapefluctuationsassociatewithchromatincondensationinearlyprophase AT giancarloporcella centripetalnuclearshapefluctuationsassociatewithchromatincondensationinearlyprophase AT pietrocicuta centripetalnuclearshapefluctuationsassociatewithchromatincondensationinearlyprophase AT marcocosentinolagomarsino centripetalnuclearshapefluctuationsassociatewithchromatincondensationinearlyprophase |