Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer

Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protoc...

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Bibliographic Details
Main Authors: Christopher D. Intemann, Thorsten Thye, Jürgen Sievertsen, Ellis Owusu-Dabo, Rolf D. Horstmann, Christian G. Meyer
Format: Article
Language:English
Published: Taylor & Francis Group 2009-01-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000113045