Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cereals
Cereal grains are widely cultivated agricultural commodities, and they constitute a key source of energy. However, many of them are affected by fungal contamination, and consequently, by the presence of mycotoxin. The development of monitoring techniques to evaluate exposure, is therefore of interes...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X23004070 |
| _version_ | 1827218736718807040 |
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| author | Filipa Carreiró Sílvia Cruz Barros Carla Brites Andreia Freitas Fernando Ramos Ana Sanches Silva |
| author_facet | Filipa Carreiró Sílvia Cruz Barros Carla Brites Andreia Freitas Fernando Ramos Ana Sanches Silva |
| author_sort | Filipa Carreiró |
| collection | DOAJ |
| description | Cereal grains are widely cultivated agricultural commodities, and they constitute a key source of energy. However, many of them are affected by fungal contamination, and consequently, by the presence of mycotoxin. The development of monitoring techniques to evaluate exposure, is therefore of interest. Immunoassays have recently emerged as potential alternatives for the study of mycotoxins. This study focuses on a validation of a biochip array technology (BAT) for multi-mycotoxins screening in oat, barley, rye, and wheat grains. The Evidence Investigator Myco 7 (RANDOX Food Diagnostic), based in a competitive chemiluminescent immunoassay, was used for the simultaneous semi-quantitative detection of the mycotoxin's immunoassays. According to validation results, spiked rice samples showed low false results (5 % of false negatives and 5 % of false positives for fumonisins, 5 % of false negatives for Zearalenone, Aflatoxin B1, T2HT2 and Deoxynivalenol and 5 % of false positives were found for ochratoxin A). In the spiked samples of other cereals, were found 5 % of false positives for fumonisin B1+fumonisin B2, zearalenone, ochratoxin A and aflatoxin B1 and 5 % of false negatives were found for Fumonisin 1 + Fumonisin 2, Ochratoxin A, Aflatoxin B1 and T2+HT2 in agreement with European Union legislation performance criteria. This BAT immunoassay provides important benefits for the rapid and efficient screening of several mycotoxins in cereal grain samples at various levels. |
| first_indexed | 2024-03-08T18:11:41Z |
| format | Article |
| id | doaj.art-9d0e2c04b7dc4574a91ff66a03db219c |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2025-03-21T15:39:39Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-9d0e2c04b7dc4574a91ff66a03db219c2024-06-19T04:47:09ZengElsevierFood Chemistry Advances2772-753X2024-06-014100586Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cerealsFilipa Carreiró0Sílvia Cruz Barros1Carla Brites2Andreia Freitas3Fernando Ramos4Ana Sanches Silva5University of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de St, Comba, 3000-548 Coimbra, Portugal; National Institute for Agrarian and Veterinary Research (INIAV), I.P., Av. da República 2780-157 Oeiras, Portugal; Centre for Animal Science Studies (CECA), ICETA, University of Porto, 4501-401 Porto, PortugalNational Institute for Agrarian and Veterinary Research (INIAV), I.P., Av. da República 2780-157 Oeiras, PortugalNational Institute for Agrarian and Veterinary Research (INIAV), I.P., Av. da República 2780-157 Oeiras, Portugal; GREEN-IT Bioresources for Sustainability, ITQB NOVA, Av. da República, 2780-157 Oeiras, PortugalNational Institute for Agrarian and Veterinary Research (INIAV), I.P., Av. da República 2780-157 Oeiras, Portugal; REQUIMTE/LAVQ, R. D. Manuel II, Apartado 55142, Porto PortugalUniversity of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de St, Comba, 3000-548 Coimbra, Portugal; REQUIMTE/LAVQ, R. D. Manuel II, Apartado 55142, Porto PortugalUniversity of Coimbra, Faculty of Pharmacy, Polo III, Azinhaga de St, Comba, 3000-548 Coimbra, Portugal; Centre for Animal Science Studies (CECA), ICETA, University of Porto, 4501-401 Porto, Portugal; Associate Laboratory for Animal and Veterinary Sciences (Al4AnimalS), 1300-477 Lisbon, Portugal; Corresponding author.Cereal grains are widely cultivated agricultural commodities, and they constitute a key source of energy. However, many of them are affected by fungal contamination, and consequently, by the presence of mycotoxin. The development of monitoring techniques to evaluate exposure, is therefore of interest. Immunoassays have recently emerged as potential alternatives for the study of mycotoxins. This study focuses on a validation of a biochip array technology (BAT) for multi-mycotoxins screening in oat, barley, rye, and wheat grains. The Evidence Investigator Myco 7 (RANDOX Food Diagnostic), based in a competitive chemiluminescent immunoassay, was used for the simultaneous semi-quantitative detection of the mycotoxin's immunoassays. According to validation results, spiked rice samples showed low false results (5 % of false negatives and 5 % of false positives for fumonisins, 5 % of false negatives for Zearalenone, Aflatoxin B1, T2HT2 and Deoxynivalenol and 5 % of false positives were found for ochratoxin A). In the spiked samples of other cereals, were found 5 % of false positives for fumonisin B1+fumonisin B2, zearalenone, ochratoxin A and aflatoxin B1 and 5 % of false negatives were found for Fumonisin 1 + Fumonisin 2, Ochratoxin A, Aflatoxin B1 and T2+HT2 in agreement with European Union legislation performance criteria. This BAT immunoassay provides important benefits for the rapid and efficient screening of several mycotoxins in cereal grain samples at various levels.http://www.sciencedirect.com/science/article/pii/S2772753X23004070MycotoxinsChemiluminescence immunoassayBiochip array technologyCerealsRice |
| spellingShingle | Filipa Carreiró Sílvia Cruz Barros Carla Brites Andreia Freitas Fernando Ramos Ana Sanches Silva Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cereals Food Chemistry Advances Mycotoxins Chemiluminescence immunoassay Biochip array technology Cereals Rice |
| title | Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cereals |
| title_full | Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cereals |
| title_fullStr | Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cereals |
| title_full_unstemmed | Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cereals |
| title_short | Validation of a biochip array technology for multi-mycotoxins screening in Rice and other cereals |
| title_sort | validation of a biochip array technology for multi mycotoxins screening in rice and other cereals |
| topic | Mycotoxins Chemiluminescence immunoassay Biochip array technology Cereals Rice |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X23004070 |
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