In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90.
Otoconia are formed embryonically and are instrumental in detecting linear acceleration and gravity. Degeneration and fragmentation of otoconia in elderly patients leads to imbalance resulting in higher frequency of falls that are positively correlated with the incidence of bone fractures and death....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3991680?pdf=render |
| _version_ | 1818874303142166528 |
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| author | K Trent Moreland Mina Hong Wenfu Lu Christopher W Rowley David M Ornitz James J De Yoreo Ruediger Thalmann |
| author_facet | K Trent Moreland Mina Hong Wenfu Lu Christopher W Rowley David M Ornitz James J De Yoreo Ruediger Thalmann |
| author_sort | K Trent Moreland |
| collection | DOAJ |
| description | Otoconia are formed embryonically and are instrumental in detecting linear acceleration and gravity. Degeneration and fragmentation of otoconia in elderly patients leads to imbalance resulting in higher frequency of falls that are positively correlated with the incidence of bone fractures and death. In this work we investigate the roles otoconial proteins Otolin-1 and Otoconin 90 (OC90) perform in the formation of otoconia. We demonstrate by rotary shadowing and atomic force microscopy (AFM) experiments that Otolin-1 forms homomeric protein complexes and self-assembled networks supporting the hypothesis that Otolin-1 serves as a scaffold protein of otoconia. Our calcium carbonate crystal growth data demonstrate that Otolin-1 and OC90 modulate in vitro calcite crystal morphology but neither protein is sufficient to produce the shape of otoconia. Coadministration of these proteins produces synergistic effects on crystal morphology that contribute to morphology resembling otoconia. |
| first_indexed | 2024-12-19T13:08:27Z |
| format | Article |
| id | doaj.art-9d1cf55c2016451b86addde4a1339fb5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T13:08:27Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9d1cf55c2016451b86addde4a1339fb52022-12-21T20:19:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9533310.1371/journal.pone.0095333In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90.K Trent MorelandMina HongWenfu LuChristopher W RowleyDavid M OrnitzJames J De YoreoRuediger ThalmannOtoconia are formed embryonically and are instrumental in detecting linear acceleration and gravity. Degeneration and fragmentation of otoconia in elderly patients leads to imbalance resulting in higher frequency of falls that are positively correlated with the incidence of bone fractures and death. In this work we investigate the roles otoconial proteins Otolin-1 and Otoconin 90 (OC90) perform in the formation of otoconia. We demonstrate by rotary shadowing and atomic force microscopy (AFM) experiments that Otolin-1 forms homomeric protein complexes and self-assembled networks supporting the hypothesis that Otolin-1 serves as a scaffold protein of otoconia. Our calcium carbonate crystal growth data demonstrate that Otolin-1 and OC90 modulate in vitro calcite crystal morphology but neither protein is sufficient to produce the shape of otoconia. Coadministration of these proteins produces synergistic effects on crystal morphology that contribute to morphology resembling otoconia.http://europepmc.org/articles/PMC3991680?pdf=render |
| spellingShingle | K Trent Moreland Mina Hong Wenfu Lu Christopher W Rowley David M Ornitz James J De Yoreo Ruediger Thalmann In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. PLoS ONE |
| title | In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. |
| title_full | In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. |
| title_fullStr | In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. |
| title_full_unstemmed | In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. |
| title_short | In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. |
| title_sort | in vitro calcite crystal morphology is modulated by otoconial proteins otolin 1 and otoconin 90 |
| url | http://europepmc.org/articles/PMC3991680?pdf=render |
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