Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation

Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation

Summary: High-throughput imaging allows in vitro assessment of neuron morphology for screening populations under developmental, homeostatic, and/or disease conditions. Here, we present a protocol to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high...

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Bibliographic Details
Main Authors: Gautam Wali, Yan Li, Dad Abu-Bonsrah, Deniz Kirik, Clare L. Parish, Carolyn M. Sue
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell Differentiation
Microscopy
Neuroscience
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002927

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http://www.sciencedirect.com/science/article/pii/S2666166723002927

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