Specific Detection of SARS-CoV-2 Variants B.1.1.7 (Alpha) and B.1.617.2 (Delta) Using a One-Step Quantitative PCR Assay

Specific Detection of SARS-CoV-2 Variants B.1.1.7 (Alpha) and B.1.617.2 (Delta) Using a One-Step Quantitative PCR Assay

ABSTRACT In this report, we describe the development of a reverse transcription-quantitative PCR (RT-qPCR) assay, termed Alpha-Delta assay, which can detect all severe acute respiratory syndrome coronavirus 2 (SC-2) variants and distinguish between the Alpha (B.1.1.7) and Delta (B.1.617.2) variants....

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Bibliographic Details
Main Authors: Oran Erster, Ella Mendelson, Areej Kabat, Virginia Levy, Batya Mannasse, Hadar Assraf, Roberto Azar, Yaniv Ali, Efrat Bucris, Dana Bar-Ilan, Orna Mor, Michal Elul, Michal Mandelboim, Danit Sofer, Shay Fleishon, Neta S. Zuckerman, Itay Bar-Or
Format: Article
Language:English
Published: American Society for Microbiology 2022-04-01
Series:Microbiology Spectrum
Subjects:
SARS-COV-2
RT-qPCR
Alpha (B.1.1.7)
Delta (B.1.617.2)
classification
diagnostic assay
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02176-21

Internet

https://journals.asm.org/doi/10.1128/spectrum.02176-21

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