Technical pitfalls when collecting, cryopreserving, thawing, and stimulating human T-cells
The collection, cryopreservation, thawing, and culture of peripheral blood mononuclear cells (PBMCs) can profoundly influence T cell viability and immunogenicity. Gold-standard PBMC processing protocols have been developed by the Office of HIV/AIDS Network Coordination (HANC); however, these protoco...
Main Authors: | , , |
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格式: | 文件 |
语言: | English |
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Frontiers Media S.A.
2024-05-01
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丛编: | Frontiers in Immunology |
主题: | |
在线阅读: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1382192/full |