Technical pitfalls when collecting, cryopreserving, thawing, and stimulating human T-cells

The collection, cryopreservation, thawing, and culture of peripheral blood mononuclear cells (PBMCs) can profoundly influence T cell viability and immunogenicity. Gold-standard PBMC processing protocols have been developed by the Office of HIV/AIDS Network Coordination (HANC); however, these protoco...

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Main Authors: Daniel J. Browne, Catherine M. Miller, Denise L. Doolan
格式: 文件
语言:English
出版: Frontiers Media S.A. 2024-05-01
丛编:Frontiers in Immunology
主题:
在线阅读:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1382192/full