Use of Flow Cytometry and RT-PCR for Detecting Gene Expression by Single Cells
We have developed a method for reliably detecting gene expression by individual, phenotypically defined cells. Cells were sorted by flow cytometry into 96-well plates containing a Nonidet® P-40 (NP40)-based lysis solution. Reverse transcription (RT) of cellular major histocompatibility complex class...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96212rr02 |