Benchmarking low- and high-throughput protein cleanup and digestion methods for human fecal metaproteomics

ABSTRACT The application of fecal metaproteomics to large-scale studies of the gut microbiota requires high-throughput analysis and standardized experimental protocols. Although high-throughput protein cleanup and digestion methods are increasingly used in shotgun proteomics, no studies have yet cri...

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Bibliographic Details
Main Authors: Alessandro Tanca, Maria Antonietta Deledda, Laura De Diego, Marcello Abbondio, Sergio Uzzau
Format: Article
Language:English
Published: American Society for Microbiology 2024-07-01
Series:mSystems
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/msystems.00661-24