Improving ethanol tolerance of ethyl carbamate hydrolase by diphasic high pressure molecular dynamic simulations
Key points 1 Diphasic high pressure molecular dynamic simulations were applied for ration engineering of ethyl carbamate hydrolase with improved ethanol tolerance. 2 The specific activity of H68A/K70R/S325N was 3.42-fold higher than WT. 3 The tolerance of triple mutant H68A/K70R/S325N to 20%(v/v) et...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-03-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-023-01538-7 |