Automation of hybridization and capture based next generation sequencing library preparation requires reduction of on-deck bead binding and heated wash temperatures

Capture-based library preparation for next generation sequencing (NGS) offers a balance between sequencing depth and bioinformatics cost of analysis. Liquid handling automation enhances the reliability of the library preparation process by reducing sample-to-sample variation and substantially enhanc...

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Bibliographic Details
Main Authors: Eric Zimmerman Zuckerman, Joseph A. Thompson, Amber R. Schneider, Michael B. Campion, Jennifer J Johns, Theodore J. Stier, Lisa M. Peterson, Amanda M. Ward, Joseph H. Blommel, Rohan D. Gnanaolivu, Kimberly P. Lauer, Gopinath Sivasankaran, Jagadheshwar Balan, Surendra Dasari, Yuta Sakai, Cherisse A. Marcou, Gang Zheng, Kevin C. Halling, Wei Shen, David S. Viswanatha, Zhiyv Niu
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:SLAS Technology
Online Access:http://www.sciencedirect.com/science/article/pii/S2472630321000169