Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat Bran
In order to take full advantage of black wheat bran in the development of whole grain functional food, the crude polyphenols extracted from black wheat bran by ultrasonic-assisted method were purified, and the macroporous resin purification process was optimized to determine the optimal purification...
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| Format: | Article |
| Language: | English |
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Academy of National Food and Strategic Reserves Administration
2024-01-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240105 |
| _version_ | 1797347157645197312 |
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| author | ZHANG Hao WANG Li LI Yan |
| author_facet | ZHANG Hao WANG Li LI Yan |
| author_sort | ZHANG Hao |
| collection | DOAJ |
| description | In order to take full advantage of black wheat bran in the development of whole grain functional food, the crude polyphenols extracted from black wheat bran by ultrasonic-assisted method were purified, and the macroporous resin purification process was optimized to determine the optimal purification conditions. Moreover, liquid chromatography and mass spectrometry (LC-MS) technology were used to analyse the components of purified polyphenols in black wheat bran. The results indicated that when the concentration of sample solution was 1.20 mg/mL, and the concentration of elution solvent was 60%, with the injection flow rate of 1.50 mL/min, and the elution rate of 1.50 mL/min, the purification effect was better. The purity of polyphenols in black wheat bran before and after purification was 2.60%±0.28% and 14.27%±0.13%, respectively. The purity of polyphenols after purification was about 5.48 times of that before purification. Nine polyphenols contained probably in the purified black wheat bran were speculated. The above research showed that the macroporous resin purification process could purify the crude polyphenol extract of black wheat bran in an effective way, and maintain the diversity of polyphenols to some extent. |
| first_indexed | 2024-03-08T11:43:37Z |
| format | Article |
| id | doaj.art-9e1fca91b4484fdd952e161e2fba508e |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-03-08T11:43:37Z |
| publishDate | 2024-01-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-9e1fca91b4484fdd952e161e2fba508e2024-01-25T02:58:12ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612024-01-01321283510.16210/j.cnki.1007-7561.2024.01.004Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat BranZHANG Hao0WANG Li1LI Yan2School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaIn order to take full advantage of black wheat bran in the development of whole grain functional food, the crude polyphenols extracted from black wheat bran by ultrasonic-assisted method were purified, and the macroporous resin purification process was optimized to determine the optimal purification conditions. Moreover, liquid chromatography and mass spectrometry (LC-MS) technology were used to analyse the components of purified polyphenols in black wheat bran. The results indicated that when the concentration of sample solution was 1.20 mg/mL, and the concentration of elution solvent was 60%, with the injection flow rate of 1.50 mL/min, and the elution rate of 1.50 mL/min, the purification effect was better. The purity of polyphenols in black wheat bran before and after purification was 2.60%±0.28% and 14.27%±0.13%, respectively. The purity of polyphenols after purification was about 5.48 times of that before purification. Nine polyphenols contained probably in the purified black wheat bran were speculated. The above research showed that the macroporous resin purification process could purify the crude polyphenol extract of black wheat bran in an effective way, and maintain the diversity of polyphenols to some extent.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240105black wheat branpolyphenolspurificationpurification process optimizationcomponent analysis |
| spellingShingle | ZHANG Hao WANG Li LI Yan Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat Bran Liang you shipin ke-ji black wheat bran polyphenols purification purification process optimization component analysis |
| title | Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat Bran |
| title_full | Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat Bran |
| title_fullStr | Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat Bran |
| title_full_unstemmed | Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat Bran |
| title_short | Purification Process Optimization and Composition Analysis of Polyphenols from Black Wheat Bran |
| title_sort | purification process optimization and composition analysis of polyphenols from black wheat bran |
| topic | black wheat bran polyphenols purification purification process optimization component analysis |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20240105 |
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