Characterization, complete genome sequencing, and CRISPR/Cas9 system-based decontamination of a novel Escherichia coli phage TR1 from fermentation substrates

Characterization, complete genome sequencing, and CRISPR/Cas9 system-based decontamination of a novel Escherichia coli phage TR1 from fermentation substrates

Phage contamination has become a major concern for industrial bacteria, such as Escherichia coli BL21(DE3), used in fermentation processes. Herein, we report a CRISPR/Cas9 defense system-based strategy to precisely prey and degrade phage DNA to decontaminate target phages. First, we isolated a novel...

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Bibliographic Details
Main Authors: Yuqi Dong, Yunfei Huang, Huahao Fan, Lihua Song, Xiaoping An, Shan Xu, Mengzhe Li, Yigang Tong
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-08-01
Series:Frontiers in Microbiology
Subjects:
fermentation
phage contamination
Escherichia coli
CRISPR/Cas9
defense system
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1230775/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2023.1230775/full

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