Established and Emerging Methods for Protecting Linear DNA in Cell-Free Expression Systems

Cell-free protein synthesis (CFPS) is a method utilized for producing proteins without the limits of cell viability. The plug-and-play utility of CFPS is a key advantage over traditional plasmid-based expression systems and is foundational to the potential of this biotechnology. A key limitation of...

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Bibliographic Details
Main Authors: Trevor J. Fochtman, Javin P. Oza
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/6/2/36