Cryopreservation of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens) shoot tips by droplet-vitrification technique
The droplet-vitrification technique was applied to in vitro shoot tips of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens). Explants were precultured in the dark at 23 °C, in liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h). Loa...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Sciendo
2013-12-01
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Series: | Journal of Horticultural Research |
Subjects: | |
Online Access: | https://doi.org/10.2478/johr-2013-0025 |