MERR APEX-seq protocol for profiling the subcellular nascent transcriptome in mammalian cells

MERR APEX-seq protocol for profiling the subcellular nascent transcriptome in mammalian cells

Summary: Knowledge about the spatial organization of RNAs in eukaryotic cells is crucial for understanding their functions. Here, we present a detailed MERR APEX-seq protocol to achieve spatiotemporally resolved mapping of the subcellular transcriptome in cultured mammalian cells. This protocol prov...

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Bibliographic Details
Main Authors: Ran Li, Peng Zou
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Sequence Analysis
Sequencing
RNAseq
Molecular Biology
Molecular/Chemical Probes
Biotechnology and Bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000151

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http://www.sciencedirect.com/science/article/pii/S2666166723000151

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