Characterisation of human embryonic stem cells conditioning media by 1H-nuclear magnetic resonance spectroscopy.

BACKGROUND: Cell culture media conditioned by human foreskin fibroblasts (HFFs) provide a complex supplement of protein and metabolic factors that support in vitro proliferation of human embryonic stem cells (hESCs). However, the conditioning process is variable with different media batches often ex...

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Bibliographic Details
Main Authors: David A MacIntyre, Darío Melguizo Sanchís, Beatriz Jiménez, Rubén Moreno, Miodrag Stojkovic, Antonio Pineda-Lucena
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3036660?pdf=render