The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases
Human classical satellite DNAs were used as probes to investigate the molecular mechanism(s) of AluI/TaqI attack in situ on specific centromeric areas. The biochemical results obtained show that the majority of such highly repetitive DNAs are not solubilized from chromosomes, in spite of a cleavage...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2009-06-01
|
| Series: | European Journal of Histochemistry |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/829 |
| _version_ | 1828404653102989312 |
|---|---|
| author | M Nieddu G Pichiri G Diaz R Mezzanotte |
| author_facet | M Nieddu G Pichiri G Diaz R Mezzanotte |
| author_sort | M Nieddu |
| collection | DOAJ |
| description | Human classical satellite DNAs were used as probes to investigate the molecular mechanism(s) of AluI/TaqI attack in situ on specific centromeric areas. The biochemical results obtained show that the majority of such highly repetitive DNAs are not solubilized from chromosomes, in spite of a cleavage pattern identical to that shown in naked genomic DNA digested with the same enzymes. Moreover, when digestion in situ with restriction enzymes precedes in situ hybridization, it is possible to observe an increased signal in the centromeres of some chromosomes as compared to that shown in standard undigested chromosomes and, on the other hand, hybridization labelling in centromeres which are difficult to detect by in situ hybridization using standard undigested chromosomes. Lastly, our results show that centromeric heterochromatin is not a homogeneous class in regard to organizational structure. |
| first_indexed | 2024-12-10T10:39:50Z |
| format | Article |
| id | doaj.art-9e94ee72c2b94908bfc7494cfaf9cf82 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-10T10:39:50Z |
| publishDate | 2009-06-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-9e94ee72c2b94908bfc7494cfaf9cf822022-12-22T01:52:20ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062009-06-0147310.4081/829564The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleasesM NiedduG PichiriG DiazR MezzanotteHuman classical satellite DNAs were used as probes to investigate the molecular mechanism(s) of AluI/TaqI attack in situ on specific centromeric areas. The biochemical results obtained show that the majority of such highly repetitive DNAs are not solubilized from chromosomes, in spite of a cleavage pattern identical to that shown in naked genomic DNA digested with the same enzymes. Moreover, when digestion in situ with restriction enzymes precedes in situ hybridization, it is possible to observe an increased signal in the centromeres of some chromosomes as compared to that shown in standard undigested chromosomes and, on the other hand, hybridization labelling in centromeres which are difficult to detect by in situ hybridization using standard undigested chromosomes. Lastly, our results show that centromeric heterochromatin is not a homogeneous class in regard to organizational structure.https://www.ejh.it/index.php/ejh/article/view/829 |
| spellingShingle | M Nieddu G Pichiri G Diaz R Mezzanotte The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases European Journal of Histochemistry |
| title | The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases |
| title_full | The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases |
| title_fullStr | The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases |
| title_full_unstemmed | The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases |
| title_short | The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases |
| title_sort | organization of classical satellite dnas in human chromosomes an approach using alui and taqi restriction endonucleases |
| url | https://www.ejh.it/index.php/ejh/article/view/829 |
| work_keys_str_mv | AT mnieddu theorganizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases AT gpichiri theorganizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases AT gdiaz theorganizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases AT rmezzanotte theorganizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases AT mnieddu organizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases AT gpichiri organizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases AT gdiaz organizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases AT rmezzanotte organizationofclassicalsatellitednasinhumanchromosomesanapproachusingaluiandtaqirestrictionendonucleases |