Correlation between Targeted qPCR Assays and Untargeted DNA Shotgun Metagenomic Sequencing for Assessing the Fecal Microbiota in Dogs

Correlation between Targeted qPCR Assays and Untargeted DNA Shotgun Metagenomic Sequencing for Assessing the Fecal Microbiota in Dogs

DNA shotgun sequencing is an untargeted approach for identifying changes in relative abundances, while qPCR allows reproducible quantification of specific bacteria. The canine dysbiosis index (DI) assesses the canine fecal microbiota by using a mathematical algorithm based on qPCR results. We evalua...

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Bibliographic Details
Main Authors: Chi-Hsuan Sung, Rachel Pilla, Chih-Chun Chen, Patricia Eri Ishii, Linda Toresson, Karin Allenspach-Jorn, Albert E. Jergens, Stacie Summers, Kelly S. Swanson, Holger Volk, Teresa Schmidt, Helene Stuebing, Johanna Rieder, Kathrin Busch, Melanie Werner, Anja Lisjak, Frederic P. Gaschen, Sara E. Belchik, M. Katherine Tolbert, Jonathan A. Lidbury, Joerg M. Steiner, Jan S. Suchodolski
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Animals
Subjects:
canine dysbiosis index
quantitative PCR
shotgun metagenomic sequencing
chronic enteropathy
microbiota
Online Access:https://www.mdpi.com/2076-2615/13/16/2597

Internet

https://www.mdpi.com/2076-2615/13/16/2597

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