OPTIMIZATION OF SODIUM DODECYL SULPHATE AS A FORMAZAN SOLVENT AND COMPARISON OF 3-(4,-5- DIMETHYLTHIAZO-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) ASSAY WITH WST-1 ASSAY IN MCF-7 CELLS

OPTIMIZATION OF SODIUM DODECYL SULPHATE AS A FORMAZAN SOLVENT AND COMPARISON OF 3-(4,-5- DIMETHYLTHIAZO-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) ASSAY WITH WST-1 ASSAY IN MCF-7 CELLS

A 3-(4,-5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is a method that used to measure cell viability. It is based on the conversion of MTT by succinic dehydrogenase enzyme into insoluble formazan. Dissolution of formazan by using proper solvent is the most important step of...

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Detaylı Bibliyografya
Asıl Yazarlar: Ratih Asmana Ningrum, Yulaika Romadhani, Popi Hadi Wisnuwardhani, Adi Santoso
Materyal Türü: Makale
Dil:English
Baskı/Yayın Bilgisi: Universitas Gadjah Mada 2014-10-01
Seri Bilgileri:Indonesian Journal of Pharmacy
Konular:
DMSO
MCF-7 cells
MTT assay
sodium dodecyl sulphate
WST-1 assay
Online Erişim:http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/274

Internet

http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/274

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