OPTIMIZATION OF SODIUM DODECYL SULPHATE AS A FORMAZAN SOLVENT AND COMPARISON OF 3-(4,-5- DIMETHYLTHIAZO-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) ASSAY WITH WST-1 ASSAY IN MCF-7 CELLS

OPTIMIZATION OF SODIUM DODECYL SULPHATE AS A FORMAZAN SOLVENT AND COMPARISON OF 3-(4,-5- DIMETHYLTHIAZO-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) ASSAY WITH WST-1 ASSAY IN MCF-7 CELLS

A 3-(4,-5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is a method that used to measure cell viability. It is based on the conversion of MTT by succinic dehydrogenase enzyme into insoluble formazan. Dissolution of formazan by using proper solvent is the most important step of...

תיאור מלא

מידע ביבליוגרפי
Main Authors: Ratih Asmana Ningrum, Yulaika Romadhani, Popi Hadi Wisnuwardhani, Adi Santoso
פורמט: Article
שפה:English
יצא לאור: Universitas Gadjah Mada 2014-10-01
סדרה:Indonesian Journal of Pharmacy
נושאים:
DMSO
MCF-7 cells
MTT assay
sodium dodecyl sulphate
WST-1 assay
גישה מקוונת:http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/274

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