Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervates
Complex coacervates of bovine lactoferrin (LF) and sodium alginate (NaAlg) were prepared aiming to protect the structure and associated function of LF in gastric stage of digestion. The parameters affecting the formation of complex coacervates (pH, LF-to-NaAlg ratio) were optimized using electrostat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617304322 |
| _version_ | 1831584730194116608 |
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| author | Bo Wang Ewan Blanch Colin J. Barrow Benu Adhikari |
| author_facet | Bo Wang Ewan Blanch Colin J. Barrow Benu Adhikari |
| author_sort | Bo Wang |
| collection | DOAJ |
| description | Complex coacervates of bovine lactoferrin (LF) and sodium alginate (NaAlg) were prepared aiming to protect the structure and associated function of LF in gastric stage of digestion. The parameters affecting the formation of complex coacervates (pH, LF-to-NaAlg ratio) were optimized using electrostatic charge density, turbidity and yield of the complex coacervates. The breaking down of LF structure into smaller peptides, antioxidant capacity and iron release/binding properties of LF and LF-NaAlg were determined in oral, gastric and intestinal stages of in-vitro digestion. The highest yield of LF-NaAlg complex coacervates was achieved at pH 4.5 and the LF-to-NaAlg ratio of 8:1. UP to 30% LF was delivered to the intestinal stage in complex coacervate form while the entire uncomplexed LF was degraded at the gastric stage. The antioxidant capacity of LF in complex coacervate was increased by 12%. The iron binding/release property of LF was not affected in LF-NaAlg complex coacervates. |
| first_indexed | 2024-12-17T21:04:40Z |
| format | Article |
| id | doaj.art-9e9ee2bb1d3d46a9b381757c54fea8c5 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T21:04:40Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-9e9ee2bb1d3d46a9b381757c54fea8c52022-12-21T21:32:37ZengElsevierJournal of Functional Foods1756-46462017-10-013797106Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervatesBo Wang0Ewan Blanch1Colin J. Barrow2Benu Adhikari3School of Science, RMIT University, Melbourne, VIC 3083, AustraliaSchool of Science, RMIT University, Melbourne, VIC 3083, AustraliaCentre for Chemistry and Biotechnology, Deakin University, Geelong, VIC 3217, AustraliaSchool of Science, RMIT University, Melbourne, VIC 3083, Australia; CSIRO Manufacturing Flagship, Clayton South, VIC 3169, Australia; Corresponding author at: School of Science, RMIT University, Melbourne, VIC 3083, Australia.Complex coacervates of bovine lactoferrin (LF) and sodium alginate (NaAlg) were prepared aiming to protect the structure and associated function of LF in gastric stage of digestion. The parameters affecting the formation of complex coacervates (pH, LF-to-NaAlg ratio) were optimized using electrostatic charge density, turbidity and yield of the complex coacervates. The breaking down of LF structure into smaller peptides, antioxidant capacity and iron release/binding properties of LF and LF-NaAlg were determined in oral, gastric and intestinal stages of in-vitro digestion. The highest yield of LF-NaAlg complex coacervates was achieved at pH 4.5 and the LF-to-NaAlg ratio of 8:1. UP to 30% LF was delivered to the intestinal stage in complex coacervate form while the entire uncomplexed LF was degraded at the gastric stage. The antioxidant capacity of LF in complex coacervate was increased by 12%. The iron binding/release property of LF was not affected in LF-NaAlg complex coacervates.http://www.sciencedirect.com/science/article/pii/S1756464617304322LactoferrinSodium alginateComplex coacervationIn-vitro digestionIron release/bindingAntioxidant capacity |
| spellingShingle | Bo Wang Ewan Blanch Colin J. Barrow Benu Adhikari Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervates Journal of Functional Foods Lactoferrin Sodium alginate Complex coacervation In-vitro digestion Iron release/binding Antioxidant capacity |
| title | Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervates |
| title_full | Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervates |
| title_fullStr | Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervates |
| title_full_unstemmed | Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervates |
| title_short | Preparation and study of digestion behavior of lactoferrin-sodium alginate complex coacervates |
| title_sort | preparation and study of digestion behavior of lactoferrin sodium alginate complex coacervates |
| topic | Lactoferrin Sodium alginate Complex coacervation In-vitro digestion Iron release/binding Antioxidant capacity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617304322 |
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