An optimized protocol for the isolation of rare stromal cell populations from the mouse spleen

Summary: Lymphoid tissue stromal cells are important regulators of spleen homeostasis and immune responses. Here, we present an optimized protocol that describes the digestion and enrichment steps for the isolation and analysis of rare populations of stromal cells, including fibroblastic reticular c...

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Bibliographic Details
Main Authors: Yannick O. Alexandre, Scott N. Mueller
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008036