Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles

Abstract The plant vacuole plays a fundamental role in cell homeostasis. The successful application of patch-clamp technique on isolated vacuoles allows the determination of the functional characteristics of tonoplast ion channels and transporters. The parallel use of a sensor-based fluorescence app...

Full description

Bibliographic Details
Main Authors: Antonella Gradogna, Armando Carpaneto
Format: Article
Language:English
Published: Springer 2022-10-01
Series:Stress Biology
Subjects:
Online Access:https://doi.org/10.1007/s44154-022-00064-z