Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles
Abstract The plant vacuole plays a fundamental role in cell homeostasis. The successful application of patch-clamp technique on isolated vacuoles allows the determination of the functional characteristics of tonoplast ion channels and transporters. The parallel use of a sensor-based fluorescence app...
Main Authors: | Antonella Gradogna, Armando Carpaneto |
---|---|
Format: | Article |
Language: | English |
Published: |
Springer
2022-10-01
|
Series: | Stress Biology |
Subjects: | |
Online Access: | https://doi.org/10.1007/s44154-022-00064-z |
Similar Items
-
The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach
by: Gradogna Antonella, et al.
Published: (2022-03-01) -
A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas
by: Minicozzi Velia, et al.
Published: (2023-09-01) -
Electrophysiological evaluation of the effect of peptide toxins on voltage-gated ion channels: a scoping review on theoretical and methodological aspects with focus on the Central and South American experience
by: Jessica Rojas-Palomino, et al.
Published: (2024-09-01) -
Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters
by: Margherita Festa, et al.
Published: (2022-03-01) -
Determination of single-molecule transport activity of OATP2B1 by measuring the number of transporter molecules using electrophysiological approach
by: Kodai Yajima, et al.
Published: (2023-11-01)