Efficient Generation of Transgenic Buffalos (Bubalus bubalis) by Nuclear Transfer of Fetal Fibroblasts Expressing Enhanced Green Fluorescent Protein

Efficient Generation of Transgenic Buffalos (Bubalus bubalis) by Nuclear Transfer of Fetal Fibroblasts Expressing Enhanced Green Fluorescent Protein

Abstract The possibility of producing transgenic cloned buffalos by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein (EGFP) was explored in this study. When buffalo fetal fibroblasts (BFFs) isolated from a male buffalo fetus were transfected with pEGFP-N1 (EGFP is...

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Bibliographic Details
Main Authors: Fenghua Lu, Chan Luo, Nan Li, Qingyou Liu, Yingming Wei, Haiying Deng, Xiaoli Wang, Xiangping Li, Jianrong Jiang, Yanfei Deng, Deshun Shi
Format: Article
Language:English
Published: Nature Portfolio 2018-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-25120-5

Internet

https://doi.org/10.1038/s41598-018-25120-5

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