Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of Vibrio cholerae and Vibrio parahaemolyticus on the BD MAX Platform

Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of Vibrio cholerae and Vibrio parahaemolyticus on the BD MAX Platform

Vibrio cholerae and Vibrio parahaemolyticus are common diarrheal pathogens of great public health concern. A multiplex TaqMan-based real-time PCR assay was developed on the BD MAX platform; this assay can simultaneously detect and differentiate V. cholerae and V. parahaemolyticus directly from human...

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Bibliographic Details
Main Authors: Zhenpeng Li, Hongxia Guan, Wei Wang, He Gao, Weihong Feng, Jie Li, Baowei Diao, Hongqun Zhao, Biao Kan, Jingyun Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Vibrio cholerae
Vibrio parahaemolyticus
multiplex
real-time PCR
BD MAX
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.639473/full

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https://www.frontiersin.org/articles/10.3389/fcimb.2021.639473/full

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