Amelioration for an ignored pitfall in reference gene selection by considering the mean expression and standard deviation of target genes

Abstract Routine tissue-specific reference genes are often used in expression studies, but target genes are not taken into account. Using the relative RT-qPCR approach, we evaluated the expression of three target genes. At the same time, meta-analyses were conducted in various ethnic groups, genders...

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Bibliographic Details
Main Authors: Ghazal Esfandiarpour, Mohammad Mokhtari, Seyed-Morteza Javadirad, Mohsen Kolahdouzan, Ahmed Almuslimawi
Format: Article
Language:English
Published: Nature Portfolio 2022-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-15277-5