Triple Reporter Assay: A Non-Overlapping Luciferase Assay for the Measurement of Complex Macromolecular Regulation in Cancer Cells Using a New Mushroom Luciferase–Luciferin Pair

Triple Reporter Assay: A Non-Overlapping Luciferase Assay for the Measurement of Complex Macromolecular Regulation in Cancer Cells Using a New Mushroom Luciferase–Luciferin Pair

This study demonstrates the development of a humanized luciferase imaging reporter based on a recently discovered mushroom luciferase (<i>Luz</i>) from <i>Neonothopanus nambi.</i> In vitro and in vivo assessments showed that human-codon-optimized <i>Luz</i> (<i...

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Bibliographic Details
Main Authors: Aaiyas Mujawar, Pratham Phadte, Ksenia A. Palkina, Nadezhda M. Markina, Ameena Mohammad, Bhushan L. Thakur, Karen S. Sarkisyan, Anastasia V. Balakireva, Pritha Ray, Ilia Yamplosky, Abhijit De
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Sensors
Subjects:
bioluminescence
fungal luciferase
triple luciferase assay
gene expression
transcriptional regulation
cancer
Online Access:https://www.mdpi.com/1424-8220/23/17/7313

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https://www.mdpi.com/1424-8220/23/17/7313

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