Strong expression of Cas9 under a new 3′-truncated TEF1α promoter enhances genome editing in Yarrowia lipolytica

Strong expression of Cas9 under a new 3′-truncated TEF1α promoter enhances genome editing in Yarrowia lipolytica

The non-conventional yeast Yarrowia lipolytica is gaining interest in biotechnology as a workhorse for the production of proteins, lipids and other biomolecules. Site-specific genome editing is however limited in this yeast. Although, this was much improved by the recent adaptation of a CRISPR-Cas9...

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Bibliographic Details
Main Authors: Benjamin Ouellet, A.M. Abdel-Mawgoud
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Current Research in Biotechnology
Subjects:
CRISPR-Cas9 Genome editing
3′truncation of promoters
5′-truncation of promoters, TEF1α
Yarrowia lipolytica
Transcription factor binding sites
Online Access:http://www.sciencedirect.com/science/article/pii/S2590262823000291

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http://www.sciencedirect.com/science/article/pii/S2590262823000291

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