Strong expression of Cas9 under a new 3′-truncated TEF1α promoter enhances genome editing in Yarrowia lipolytica
The non-conventional yeast Yarrowia lipolytica is gaining interest in biotechnology as a workhorse for the production of proteins, lipids and other biomolecules. Site-specific genome editing is however limited in this yeast. Although, this was much improved by the recent adaptation of a CRISPR-Cas9...
Main Authors: | Benjamin Ouellet, A.M. Abdel-Mawgoud |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-01-01
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Series: | Current Research in Biotechnology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2590262823000291 |
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