MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway.
IL-2 plays a key role in the survival and proliferation of immune cells, especially T lymphocytes. Its expression is precisely regulated at transcriptional and posttranscriptional level. IL-2 is known to be regulated by RNA binding proteins, such as tristetraprolin (TTP), via an AU-rich element (ARE...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3504106?pdf=render |
| _version_ | 1811197848750915584 |
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| author | Min Li Wenqiang Cao Haifeng Liu Wei Zhang Xia Liu Zhijian Cai Jing Guo Xuelian Wang Zhaoyuan Hui Hang Zhang Jianli Wang Lie Wang |
| author_facet | Min Li Wenqiang Cao Haifeng Liu Wei Zhang Xia Liu Zhijian Cai Jing Guo Xuelian Wang Zhaoyuan Hui Hang Zhang Jianli Wang Lie Wang |
| author_sort | Min Li |
| collection | DOAJ |
| description | IL-2 plays a key role in the survival and proliferation of immune cells, especially T lymphocytes. Its expression is precisely regulated at transcriptional and posttranscriptional level. IL-2 is known to be regulated by RNA binding proteins, such as tristetraprolin (TTP), via an AU-rich element (ARE) in the 3'-untranslated region (3'UTR) to influence the stability of mRNA. MCPIP1, identified as a novel RNase, can degrade IL-6, IL-12 and TNF-α mRNA by an ARE-independent pathway in the activation of macrophages. Here, we reported that MCPIP1 was induced in the activation of T lymphocytes and negatively regulated IL-2 gene expression in both mouse and human primary T lymphocytes through destabilizing its mRNA. A set of Luciferase reporter assay demonstrated that a non-ARE conserved element in IL-2 3'UTR, which formed a stem-loop structure, responded to MCPIP1 activity.RNA immunoprecipitation and Biotin pulldown experiments further suggested that MCPIP1 could modestly bind to IL-2 mRNA. Taken together, these data demonstrate that MCPIP1 down-regulates IL-2 via an ARE-independent pathway. |
| first_indexed | 2024-04-12T01:21:20Z |
| format | Article |
| id | doaj.art-9f86cb674ecc4ed9936874ccf7426955 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T01:21:20Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9f86cb674ecc4ed9936874ccf74269552022-12-22T03:53:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4984110.1371/journal.pone.0049841MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway.Min LiWenqiang CaoHaifeng LiuWei ZhangXia LiuZhijian CaiJing GuoXuelian WangZhaoyuan HuiHang ZhangJianli WangLie WangIL-2 plays a key role in the survival and proliferation of immune cells, especially T lymphocytes. Its expression is precisely regulated at transcriptional and posttranscriptional level. IL-2 is known to be regulated by RNA binding proteins, such as tristetraprolin (TTP), via an AU-rich element (ARE) in the 3'-untranslated region (3'UTR) to influence the stability of mRNA. MCPIP1, identified as a novel RNase, can degrade IL-6, IL-12 and TNF-α mRNA by an ARE-independent pathway in the activation of macrophages. Here, we reported that MCPIP1 was induced in the activation of T lymphocytes and negatively regulated IL-2 gene expression in both mouse and human primary T lymphocytes through destabilizing its mRNA. A set of Luciferase reporter assay demonstrated that a non-ARE conserved element in IL-2 3'UTR, which formed a stem-loop structure, responded to MCPIP1 activity.RNA immunoprecipitation and Biotin pulldown experiments further suggested that MCPIP1 could modestly bind to IL-2 mRNA. Taken together, these data demonstrate that MCPIP1 down-regulates IL-2 via an ARE-independent pathway.http://europepmc.org/articles/PMC3504106?pdf=render |
| spellingShingle | Min Li Wenqiang Cao Haifeng Liu Wei Zhang Xia Liu Zhijian Cai Jing Guo Xuelian Wang Zhaoyuan Hui Hang Zhang Jianli Wang Lie Wang MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway. PLoS ONE |
| title | MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway. |
| title_full | MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway. |
| title_fullStr | MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway. |
| title_full_unstemmed | MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway. |
| title_short | MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway. |
| title_sort | mcpip1 down regulates il 2 expression through an are independent pathway |
| url | http://europepmc.org/articles/PMC3504106?pdf=render |
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