Rapid Detection of SARS-CoV-2 Using Duplex Reverse Transcription-Multienzyme Isothermal Rapid Amplification in a Point-of-Care Testing

Rapid Detection of SARS-CoV-2 Using Duplex Reverse Transcription-Multienzyme Isothermal Rapid Amplification in a Point-of-Care Testing

In December 2019, a severe acute respiratory syndrome caused by SARS-CoV-2 spread rapidly worldwide. Portable nucleic acid tests of SARS-CoV-2 are critically important for diagnostics. In this study, we used an isothermal amplification method—Multienzyme Isothermal Rapid Amplification (MIRA)—for rap...

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Bibliographic Details
Main Authors: Hui Chen, Chang Sun, Yang Wang, Xiaojiao Gao, Jinwei You, Wanwan Yu, Ning Sun, Yang Yang, Xiaojun Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-10-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
SARS-CoV-2
duplex multienzyme isothermal rapid amplification
point-of-care testing
lateral flow dipstick
nucleic acid tests
without RNA extraction
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.678703/full

Internet

https://www.frontiersin.org/articles/10.3389/fcimb.2021.678703/full

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