Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data

Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data

The most realistic information about a transparent sample such as a live cell can be obtained using bright-field light microscopy. Under high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response from an observed sample using a bright-field microscope equipped w...

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Bibliographic Details
Main Authors: Ganna Platonova, Dalibor Štys, Pavel Souček, Kirill Lonhus, Jan Valenta, Renata Rychtáriková
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Photonics
Subjects:
bright-field photon transmission microscope
12-bit raw data
colorimetric calibration
least information loss conversion
video-enhanced microscopy
microscope images correction
Online Access:https://www.mdpi.com/2304-6732/8/8/333

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https://www.mdpi.com/2304-6732/8/8/333

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