High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells

High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells

CRISPR/Cas9 technology has the potential to significantly enhance plant breeding. To determine the specificity and the mutagenic spectrum of SpCas9 in tomato, we designed 89 g(uide) RNAs targeting genes of the tomato MYB transcription factor family with varying predicted specificities. Plasmids enco...

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Bibliographic Details
Main Authors: Ellen Slaman, Michiel Lammers, Gerco C. Angenent, Ruud A. de Maagd
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Genome Editing
Subjects:
CRISPR-Cas9
DNA repair
CRISPR specificity
tomato
protoplast
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2023.1196763/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2023.1196763/full

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