Affinity capillary electrophoresis – mass spectrometry permits direct binding assessment of IgG and FcγRIIa in a glycoform-resolved manner

Affinity capillary electrophoresis – mass spectrometry permits direct binding assessment of IgG and FcγRIIa in a glycoform-resolved manner

The impact of antibody glycoforms on FcγRIIa activation and immune responses is poorly understood. Yet, glycoform binding assessment remains one of the major analytical challenges requiring long enrichment or glycoengineering steps. Here, we developed and applied an affinity capillary electrophoresi...

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Bibliographic Details
Main Authors: Christoph Gstöttner, Alexander Knaupp, Gestur Vidarsson, Dietmar Reusch, Tilman Schlothauer, Manfred Wuhrer, Elena Domínguez-Vega
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-09-01
Series:Frontiers in Immunology
Subjects:
affinity capillary electrophoresis (ACE)
mass spectrometry
FcγRIIa receptor
monoclonal antibody
glycosylation
interaction
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2022.980291/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2022.980291/full

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