Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate Signaling
Heart failure (HF) increases the probability of cardiac arrhythmias, including atrial fibrillation (AF), but the mechanisms linking HF to AF are poorly understood. We investigated disturbances in Ca<sup>2+</sup> signaling and electrophysiology in rabbit atrial myocytes from normal and fa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/1/53 |
| _version_ | 1797344564931985408 |
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| author | Giedrius Kanaporis Lothar A. Blatter |
| author_facet | Giedrius Kanaporis Lothar A. Blatter |
| author_sort | Giedrius Kanaporis |
| collection | DOAJ |
| description | Heart failure (HF) increases the probability of cardiac arrhythmias, including atrial fibrillation (AF), but the mechanisms linking HF to AF are poorly understood. We investigated disturbances in Ca<sup>2+</sup> signaling and electrophysiology in rabbit atrial myocytes from normal and failing hearts and identified mechanisms that contribute to the higher risk of atrial arrhythmias in HF. Ca<sup>2+</sup> transient (CaT) alternans—beat-to-beat alternations in CaT amplitude—served as indicator of increased arrhythmogenicity. We demonstrate that HF atrial myocytes were more prone to alternans despite no change in action potentials duration and only moderate decrease of L-type Ca<sup>2+</sup> current. Ca<sup>2+</sup>/calmodulin-dependent kinase II (CaMKII) inhibition suppressed CaT alternans. Activation of IP<sub>3</sub> signaling by endothelin-1 (ET-1) and angiotensin II (Ang II) resulted in acute, but transient reduction of CaT amplitude and sarcoplasmic reticulum (SR) Ca<sup>2+</sup> load, and lowered the alternans risk. However, prolonged exposure to ET-1 and Ang II enhanced SR Ca<sup>2+</sup> release and increased the degree of alternans. Inhibition of IP<sub>3</sub> receptors prevented the transient ET-1 and Ang II effects and by itself increased the degree of CaT alternans. Our data suggest that activation of CaMKII and IP<sub>3</sub> signaling contribute to atrial arrhythmogenesis in HF. |
| first_indexed | 2024-03-08T11:04:34Z |
| format | Article |
| id | doaj.art-9fea869bafcd4eb2b95ac7d37e83d1a3 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-08T11:04:34Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9fea869bafcd4eb2b95ac7d37e83d1a32024-01-26T15:18:51ZengMDPI AGBiomolecules2218-273X2023-12-011415310.3390/biom14010053Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate SignalingGiedrius Kanaporis0Lothar A. Blatter1Department of Physiology & Biophysics, Rush University Medical Center, Chicago, IL 60612, USADepartment of Physiology & Biophysics, Rush University Medical Center, Chicago, IL 60612, USAHeart failure (HF) increases the probability of cardiac arrhythmias, including atrial fibrillation (AF), but the mechanisms linking HF to AF are poorly understood. We investigated disturbances in Ca<sup>2+</sup> signaling and electrophysiology in rabbit atrial myocytes from normal and failing hearts and identified mechanisms that contribute to the higher risk of atrial arrhythmias in HF. Ca<sup>2+</sup> transient (CaT) alternans—beat-to-beat alternations in CaT amplitude—served as indicator of increased arrhythmogenicity. We demonstrate that HF atrial myocytes were more prone to alternans despite no change in action potentials duration and only moderate decrease of L-type Ca<sup>2+</sup> current. Ca<sup>2+</sup>/calmodulin-dependent kinase II (CaMKII) inhibition suppressed CaT alternans. Activation of IP<sub>3</sub> signaling by endothelin-1 (ET-1) and angiotensin II (Ang II) resulted in acute, but transient reduction of CaT amplitude and sarcoplasmic reticulum (SR) Ca<sup>2+</sup> load, and lowered the alternans risk. However, prolonged exposure to ET-1 and Ang II enhanced SR Ca<sup>2+</sup> release and increased the degree of alternans. Inhibition of IP<sub>3</sub> receptors prevented the transient ET-1 and Ang II effects and by itself increased the degree of CaT alternans. Our data suggest that activation of CaMKII and IP<sub>3</sub> signaling contribute to atrial arrhythmogenesis in HF.https://www.mdpi.com/2218-273X/14/1/53heart failureatriaalternanscalciumCa<sup>2+</sup>/calmodulin-dependent kinase IIarrhythmia |
| spellingShingle | Giedrius Kanaporis Lothar A. Blatter Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate Signaling Biomolecules heart failure atria alternans calcium Ca<sup>2+</sup>/calmodulin-dependent kinase II arrhythmia |
| title | Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate Signaling |
| title_full | Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate Signaling |
| title_fullStr | Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate Signaling |
| title_full_unstemmed | Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate Signaling |
| title_short | Increased Risk for Atrial Alternans in Rabbit Heart Failure: The Role of Ca<sup>2+</sup>/Calmodulin-Dependent Kinase II and Inositol-1,4,5-trisphosphate Signaling |
| title_sort | increased risk for atrial alternans in rabbit heart failure the role of ca sup 2 sup calmodulin dependent kinase ii and inositol 1 4 5 trisphosphate signaling |
| topic | heart failure atria alternans calcium Ca<sup>2+</sup>/calmodulin-dependent kinase II arrhythmia |
| url | https://www.mdpi.com/2218-273X/14/1/53 |
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