Immunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quails
Abstract Aim This study evaluated the effect of co-administration of vitamin C and Arabic gum (AG) supplements on the response of vaccinated (VAC) and challenged laying Japanese quails with avian influenza virus (AIV) H9N2. Materials and methods One hundred and fifty 49-day-old laying Japanese quail...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03495-y |
| _version_ | 1797983912678391808 |
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| author | Abdelfattah H. Eladl Verginia M. Farag Reham A. El-Shafei Abeer E. Aziza Walaa F. Awadin Nagah Arafat |
| author_facet | Abdelfattah H. Eladl Verginia M. Farag Reham A. El-Shafei Abeer E. Aziza Walaa F. Awadin Nagah Arafat |
| author_sort | Abdelfattah H. Eladl |
| collection | DOAJ |
| description | Abstract Aim This study evaluated the effect of co-administration of vitamin C and Arabic gum (AG) supplements on the response of vaccinated (VAC) and challenged laying Japanese quails with avian influenza virus (AIV) H9N2. Materials and methods One hundred and fifty 49-day-old laying Japanese quails were divided into 5 groups (G1-G5): the G1 group was a negative control, G2 group was unvaccinated + H9N2 challenged (Ch), G3 group was unvaccinated + supplements + Ch, G4 group was VAC + Ch, and the G5 group was VAC + supplements + Ch. The supplements (vitamin C, 1 g/liter of drinking water and AG, 1% ration) were given for 5 weeks post-vaccination (PV). The birds were injected subcutaneously with an inactivated H9N2 vaccine at 49 days of age. The quails were then challenged intranasally with AIV H9N2 at the 3rd week PV. Blood, tracheal swab and tissue samples were collected at the 1st, 2nd, and 3rd weeks PV, and at different time points post-challenge (PC). Results Growth performance, egg production (%), egg and eggshell weights, HI antibody titers, clinical signs, lesions, mortality, virus shedding rates, leukogram, biochemical and immunological parameters and histopathological lesions PC showed significant differences (P < 0.05) between the vaccinated-unsupplemented (G4) group and the vaccinated-supplemented (G5) group. G5 showed the highest (P < 0.05) growth performance, egg production, HI antibody titers, and heterophil phagocytic activity and the lowest heterophil/lymphocyte (H/L) ratio, mortality, virus shedding rates, creatinine level and histopathological lesion scores in the lungs. Conclusion The co-administration of vitamin C and AG for 5 weeks can improve growth performance, egg production and the immune response in vaccinated laying quails challenged with AIV H9N2. |
| first_indexed | 2024-04-11T06:53:41Z |
| format | Article |
| id | doaj.art-a0099a7c3e2c437297b124d0f582a605 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-11T06:53:41Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-a0099a7c3e2c437297b124d0f582a6052022-12-22T04:39:05ZengBMCBMC Veterinary Research1746-61482022-11-0118111410.1186/s12917-022-03495-yImmunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quailsAbdelfattah H. Eladl0Verginia M. Farag1Reham A. El-Shafei2Abeer E. Aziza3Walaa F. Awadin4Nagah Arafat5Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Pharmacology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Nutrition, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Pathology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura UniversityAbstract Aim This study evaluated the effect of co-administration of vitamin C and Arabic gum (AG) supplements on the response of vaccinated (VAC) and challenged laying Japanese quails with avian influenza virus (AIV) H9N2. Materials and methods One hundred and fifty 49-day-old laying Japanese quails were divided into 5 groups (G1-G5): the G1 group was a negative control, G2 group was unvaccinated + H9N2 challenged (Ch), G3 group was unvaccinated + supplements + Ch, G4 group was VAC + Ch, and the G5 group was VAC + supplements + Ch. The supplements (vitamin C, 1 g/liter of drinking water and AG, 1% ration) were given for 5 weeks post-vaccination (PV). The birds were injected subcutaneously with an inactivated H9N2 vaccine at 49 days of age. The quails were then challenged intranasally with AIV H9N2 at the 3rd week PV. Blood, tracheal swab and tissue samples were collected at the 1st, 2nd, and 3rd weeks PV, and at different time points post-challenge (PC). Results Growth performance, egg production (%), egg and eggshell weights, HI antibody titers, clinical signs, lesions, mortality, virus shedding rates, leukogram, biochemical and immunological parameters and histopathological lesions PC showed significant differences (P < 0.05) between the vaccinated-unsupplemented (G4) group and the vaccinated-supplemented (G5) group. G5 showed the highest (P < 0.05) growth performance, egg production, HI antibody titers, and heterophil phagocytic activity and the lowest heterophil/lymphocyte (H/L) ratio, mortality, virus shedding rates, creatinine level and histopathological lesion scores in the lungs. Conclusion The co-administration of vitamin C and AG for 5 weeks can improve growth performance, egg production and the immune response in vaccinated laying quails challenged with AIV H9N2.https://doi.org/10.1186/s12917-022-03495-yVitamin CArabic gumAIV H9N2VaccineLaying quailsImmune response |
| spellingShingle | Abdelfattah H. Eladl Verginia M. Farag Reham A. El-Shafei Abeer E. Aziza Walaa F. Awadin Nagah Arafat Immunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quails BMC Veterinary Research Vitamin C Arabic gum AIV H9N2 Vaccine Laying quails Immune response |
| title | Immunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quails |
| title_full | Immunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quails |
| title_fullStr | Immunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quails |
| title_full_unstemmed | Immunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quails |
| title_short | Immunological, biochemical and pathological effects of vitamin C and Arabic gum co-administration on H9N2 avian influenza virus vaccinated and challenged laying Japanese quails |
| title_sort | immunological biochemical and pathological effects of vitamin c and arabic gum co administration on h9n2 avian influenza virus vaccinated and challenged laying japanese quails |
| topic | Vitamin C Arabic gum AIV H9N2 Vaccine Laying quails Immune response |
| url | https://doi.org/10.1186/s12917-022-03495-y |
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