Fusion primer driven racket PCR: A novel tool for genome walking

Fusion primer driven racket PCR: A novel tool for genome walking

The limitations of the current genome-walking strategies include strong background and cumbersome experimental processes. Herein, we report a genome-walking method, fusion primer-driven racket PCR (FPR-PCR), for the reliable retrieval of unknown flanking DNA sequences. Four sequence-specific primers...

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Bibliographic Details
Main Authors: Jinfeng Pei, Tianyi Sun, Lingqin Wang, Zhenkang Pan, Xinyue Guo, Haixing Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Genetics
Subjects:
fusion primer
walking primer
partial annealing
racket-like DNA
genome-walking
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2022.969840/full

Internet

https://www.frontiersin.org/articles/10.3389/fgene.2022.969840/full

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