An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging

Abstract Phototoxicity is an important issue in fluorescence live imaging of light-sensitive cellular processes such as mitosis. Among several approaches to reduce phototoxicity, the addition of antioxidants to the media has been used as a simple method. Here, we analyzed the impact of phototoxicity...

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Bibliographic Details
Main Authors: Tomoki Harada, Shoji Hata, Rioka Takagi, Takuma Komori, Masamitsu Fukuyama, Takumi Chinen, Daiju Kitagawa
Format: Article
Language:English
Published: Nature Portfolio 2023-11-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-023-05479-6