Engineering efficient termination of bacteriophage T7 RNA polymerase transcription
AbstractThe bacteriophage T7 expression system is one of the most prominent transcription systems used in biotechnology and molecular-level research. However, T7 RNA polymerase is prone to read-through transcription due to its high processivity. As a consequence, enforcing efficient...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Oxford University Press
2022-03-01
|
Series: | G3: Genes, Genomes, Genetics |
Online Access: | https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkac070 |