Engineering efficient termination of bacteriophage T7 RNA polymerase transcription

AbstractThe bacteriophage T7 expression system is one of the most prominent transcription systems used in biotechnology and molecular-level research. However, T7 RNA polymerase is prone to read-through transcription due to its high processivity. As a consequence, enforcing efficient...

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Bibliographic Details
Main Authors: Diana G Calvopina-Chavez, Mikaela A Gardner, Joel S Griffitts
Format: Article
Language:English
Published: Oxford University Press 2022-03-01
Series:G3: Genes, Genomes, Genetics
Online Access:https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkac070