Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo
The protective effect of water extracts of Djulis (Chenopodium formosanum) (WECF) and its bioactive compounds against carbon tetrachloride-induced liver injury in rats was investigated. Rutin, kaempferol, betanin and another nine compounds were present in WECF using HPLC-DAD and HPLC-MS/MS analyses....
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616302390 |
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| author | Chin-Chen Chu Shih-Ying Chen Charng-Cherng Chyau Zi-Han Fu Ching-Chih Liu Pin-Der Duh |
| author_facet | Chin-Chen Chu Shih-Ying Chen Charng-Cherng Chyau Zi-Han Fu Ching-Chih Liu Pin-Der Duh |
| author_sort | Chin-Chen Chu |
| collection | DOAJ |
| description | The protective effect of water extracts of Djulis (Chenopodium formosanum) (WECF) and its bioactive compounds against carbon tetrachloride-induced liver injury in rats was investigated. Rutin, kaempferol, betanin and another nine compounds were present in WECF using HPLC-DAD and HPLC-MS/MS analyses. Oral administration of WECF to rats at 2.5 mg/kg bw for 28 consecutive days before a single dose of CCl4 demonstrated significantly lowered aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, and attenuated histopathological changes in CCl4-treated rats. WECF inhibited lipid peroxidation, restored glutathione (GSH), enhanced superoxide dismutase (SOD), and reduced DNA damage in CCl4-treated rats. Rutin, kaempferol and betanin at 1.0 µg/kg bw restored GSH and reduced DNA damage in CCl4-treated rats. In addition, betanin increased SOD activity. Overall, WECF protects rat liver from CCl4-treated liver injury due mainly to attenuating oxidative stress. The presence of bioactive compounds in WECF may partly be responsible for the hepatoprotection of WECF. |
| first_indexed | 2024-12-24T11:20:10Z |
| format | Article |
| id | doaj.art-a054fc5767674473980f4035dda0dda8 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T11:20:10Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-a054fc5767674473980f4035dda0dda82022-12-21T16:58:16ZengElsevierJournal of Functional Foods1756-46462016-10-0126585597Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivoChin-Chen Chu0Shih-Ying Chen1Charng-Cherng Chyau2Zi-Han Fu3Ching-Chih Liu4Pin-Der Duh5Department of Anesthesiology, Chi Mei Medical Center, Tainan, Taiwan, ROC; Department of Recreation and Health-Care Management, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, ROCDepartment of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, ROCResearch Institute of Biotechnology, Hungkuang University, 34 Chung-Chie Road, Shalu County, Taichung, Taiwan, ROCDepartment of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, ROCDepartment of Ophthalmology, Chi Mei Medical Center, Tainan, Taiwan, ROCDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan, ROC; Corresponding author. Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan, ROC. Fax: 886 6 3663882.The protective effect of water extracts of Djulis (Chenopodium formosanum) (WECF) and its bioactive compounds against carbon tetrachloride-induced liver injury in rats was investigated. Rutin, kaempferol, betanin and another nine compounds were present in WECF using HPLC-DAD and HPLC-MS/MS analyses. Oral administration of WECF to rats at 2.5 mg/kg bw for 28 consecutive days before a single dose of CCl4 demonstrated significantly lowered aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, and attenuated histopathological changes in CCl4-treated rats. WECF inhibited lipid peroxidation, restored glutathione (GSH), enhanced superoxide dismutase (SOD), and reduced DNA damage in CCl4-treated rats. Rutin, kaempferol and betanin at 1.0 µg/kg bw restored GSH and reduced DNA damage in CCl4-treated rats. In addition, betanin increased SOD activity. Overall, WECF protects rat liver from CCl4-treated liver injury due mainly to attenuating oxidative stress. The presence of bioactive compounds in WECF may partly be responsible for the hepatoprotection of WECF.http://www.sciencedirect.com/science/article/pii/S1756464616302390Djulis (Chenopodium formosanum)HepatoprotectionOxidative stressSuperoxide dismutaseGlutathioneBioactive compounds |
| spellingShingle | Chin-Chen Chu Shih-Ying Chen Charng-Cherng Chyau Zi-Han Fu Ching-Chih Liu Pin-Der Duh Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo Journal of Functional Foods Djulis (Chenopodium formosanum) Hepatoprotection Oxidative stress Superoxide dismutase Glutathione Bioactive compounds |
| title | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo |
| title_full | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo |
| title_fullStr | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo |
| title_full_unstemmed | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo |
| title_short | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo |
| title_sort | protective effect of djulis chenopodium formosanum and its bioactive compounds against carbon tetrachloride induced liver injury in vivo |
| topic | Djulis (Chenopodium formosanum) Hepatoprotection Oxidative stress Superoxide dismutase Glutathione Bioactive compounds |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616302390 |
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