Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks
Objective This study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet. Methods Five healthy small-tailed Han ewes with permanent rumen fistula w...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2024-04-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-23-0213.pdf |
| _version_ | 1797208805795168256 |
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| author | Huan Wei Jiancheng Liu Mengjian Liu Huiling Zhang Yong Chen |
| author_facet | Huan Wei Jiancheng Liu Mengjian Liu Huiling Zhang Yong Chen |
| author_sort | Huan Wei |
| collection | DOAJ |
| description | Objective This study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet. Methods Five healthy small-tailed Han ewes with permanent rumen fistula were selected and housed in individual pens. This study adopted a self-controlled and crossover design with a control period and an experimental period. During the control period, the animals were fed a basal diet (with a ratio of concentrate to roughage of 65:35), while during the treatment period, the animals were fed the basal diet supplemented with 0.5% hydroethanolic extract of WGH. Fermentation parameters, digestive enzyme activities, and microbial diversity in rumen fluid were analyzed. Results Supplementation of hydroethanolic extract of WGH had no significant effect on feed intake, concentrations of total volatile fatty acids, isovalerate, ammonia nitrogen, and microbial protein (p>0.05). However, the ruminal pH, concentrations of acetate, butyrate and isobutyrate, the ratio of acetate to propionate, protozoa count, and the activities of filter paper cellulase and cellobiase were significantly increased (p<0.05), while concentrations of propionate and valerate were significantly decreased (p<0.05). Moreover, 16S rRNA gene sequencing revealed that the relative abundance of rumen bacteria Christensenellaceae R7 group, Saccharofermentans, and Ruminococcaceae NK4A214 group were significantly increased, while Ruminococcus gauvreauii group, Prevotella 7 were significantly decreased (p<0.05). The relative abundance of the fungus Pseudomonas significantly increased, while Basidiomycota, Fusarium, and Alternaria significantly decreased (p<0.05). However, there was no significant change in the community structure of methanogenic archaea. Conclusion Supplementation of hydroethanolic extract of WGH to a high-concentrate diet improved the ruminal fermentation, altered the structure of ruminal bacterial and fungal communities, and exhibited beneficial effects in alleviating subacute rumen acidosis of sheep. |
| first_indexed | 2024-03-07T14:30:17Z |
| format | Article |
| id | doaj.art-a06b7850313f470e8040d8024d374aaa |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-24T09:44:39Z |
| publishDate | 2024-04-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
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| series | Animal Bioscience |
| spelling | doaj.art-a06b7850313f470e8040d8024d374aaa2024-04-15T04:05:41ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352024-04-0137465566710.5713/ab.23.021325149Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husksHuan Wei0Jiancheng Liu1Mengjian Liu2Huiling Zhang3Yong Chen4 Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China Laboratory of Nutrition for Meat & Dairy Herbivore, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, ChinaObjective This study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet. Methods Five healthy small-tailed Han ewes with permanent rumen fistula were selected and housed in individual pens. This study adopted a self-controlled and crossover design with a control period and an experimental period. During the control period, the animals were fed a basal diet (with a ratio of concentrate to roughage of 65:35), while during the treatment period, the animals were fed the basal diet supplemented with 0.5% hydroethanolic extract of WGH. Fermentation parameters, digestive enzyme activities, and microbial diversity in rumen fluid were analyzed. Results Supplementation of hydroethanolic extract of WGH had no significant effect on feed intake, concentrations of total volatile fatty acids, isovalerate, ammonia nitrogen, and microbial protein (p>0.05). However, the ruminal pH, concentrations of acetate, butyrate and isobutyrate, the ratio of acetate to propionate, protozoa count, and the activities of filter paper cellulase and cellobiase were significantly increased (p<0.05), while concentrations of propionate and valerate were significantly decreased (p<0.05). Moreover, 16S rRNA gene sequencing revealed that the relative abundance of rumen bacteria Christensenellaceae R7 group, Saccharofermentans, and Ruminococcaceae NK4A214 group were significantly increased, while Ruminococcus gauvreauii group, Prevotella 7 were significantly decreased (p<0.05). The relative abundance of the fungus Pseudomonas significantly increased, while Basidiomycota, Fusarium, and Alternaria significantly decreased (p<0.05). However, there was no significant change in the community structure of methanogenic archaea. Conclusion Supplementation of hydroethanolic extract of WGH to a high-concentrate diet improved the ruminal fermentation, altered the structure of ruminal bacterial and fungal communities, and exhibited beneficial effects in alleviating subacute rumen acidosis of sheep.http://www.animbiosci.org/upload/pdf/ab-23-0213.pdfhydroethanolic extractmicrobial communityrumen fermentationsubacute rumen acidosisvolatile fatty acidswalnut green husks |
| spellingShingle | Huan Wei Jiancheng Liu Mengjian Liu Huiling Zhang Yong Chen Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks Animal Bioscience hydroethanolic extract microbial community rumen fermentation subacute rumen acidosis volatile fatty acids walnut green husks |
| title | Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks |
| title_full | Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks |
| title_fullStr | Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks |
| title_full_unstemmed | Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks |
| title_short | Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks |
| title_sort | rumen fermentation and microbial diversity of sheep fed a high concentrate diet supplemented with hydroethanolic extract of walnut green husks |
| topic | hydroethanolic extract microbial community rumen fermentation subacute rumen acidosis volatile fatty acids walnut green husks |
| url | http://www.animbiosci.org/upload/pdf/ab-23-0213.pdf |
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